FastQCFastQC Report
Wed 4 Dec 2019
SRR3180535_EtOH1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3180535_EtOH1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54952527
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTCCATCTCGTATG3671990.6682113090085922TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA445500.040.06781
ACACGTC496200.039.871813
CTCGTAT489850.039.21998643
TCTCGTA494300.038.8354942
TCGTATG495300.038.7669244
CGTCTGA520750.037.97572316
CACACGT523800.037.7876112
ATCGGAA473650.037.6348152
CACGTCT539550.036.6968814
GCACACG545200.036.48194511
ACGTCTG543800.036.3619415
ATCTCGT537000.035.8432841
CATCTCG571100.034.09235440
CGGAAGA593100.033.6145364
TCGGAAG538550.033.315653
GTCTGAA625050.031.77580817
AGAGCAC636100.031.624078
AGCACAC643100.031.16081410
GAGCACA650400.031.0979869
TCTGAAC638100.031.08472318
Produced by FastQC (version 0.11.4)