Precourse preparations

On top of a thirst for knowledge, and a working Internet connection, here is what you will need for the course :

NGS

We expect participants to already have a basic knowledge in Next Generation Sequencing (NGS) techniques.

R

If you are not familiar with R, we will provide a short crash course during the half-day before the beginning of the course.

A basic knowledge of the R language is required to perform most analytical steps after reads have been mapped and quantified : differential gene expression, gene set enrichment, over-representation/pathway analysis.

If you want to learn R on your own, we recommend the SIB First Steps with R course, or you can pick one among this list

UNIX

If you are not familiar with the UNIX command line, we will provide a short crash course during the half-day before the beginning of the course.

Practical knowledge of the UNIX command line is also required to be able to follow this course, given that that the tools used to process sequenced reads use this interface.

If you are unsure about your capabilities or feel a bit rusty, we strongly recommend you spend some time practicing before the course : in our experience, the more comfortable you are with UNIX, the more you will be able to focus on the RNA-seq during the course, and the more you will gain from it.

If you want to learn the UNIX command line on your own, you may refer to the SIB’s UNIX e-learning module